Counting of escherichia coli by a microflow cytometer based on a photonic-microfluidic integrated device

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DOIResolve DOI: http://doi.org/10.1002/elps.201400211
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TypeArticle
Journal titleElectrophoresis
ISSN0173-0835
Volume36
Issue2
Pages298304; # of pages: 7
Subjectpolystyrene; analytical equipment; bacterial count; bacterial strain; bacterium culture; bacterium detection; biochip; concentration (parameters); Escherichia coli; flow cytometer; flow kinetics; hemocytometer; laboratory device; light scattering; microfluidics; particle size; photonic microfluidic integrated device; signal detection; suspension
AbstractCounting of Escherichia coli DH5α-cell suspensions in PBS is performed using a microflow cytometer based on a photonic-microfluidic integrated device. Side-scattered light signals are used to count the E. coli cells. A detection efficiency of 92% is achieved when compared with the expected count from a hemocytometer. The detection efficiency is correlated to the ratio of sample to sheath flow rates. It is demonstrated that E. coli can be easily distinguished from beads of similar sizes (2-4 μm) as their scattering intensities are different.
Publication date
PublisherWiley
LanguageEnglish
AffiliationNational Research Council Canada (NRC-CNRC); Information and Communication Technologies
Peer reviewedYes
NPARC number21275809
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Record identifierfcc2ed3e-a1ef-4236-a4e9-c81da5bcfdc6
Record created2015-07-14
Record modified2016-05-09
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