Ultrastructure of a bio-electrolytic methanogenic-methanotrophic granular biofilm for the complete degradation of tetrachloroethylene in contaminated groundwater

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DOIResolve DOI: http://doi.org/10.2166/wst.2007.292
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TypeArticle
Journal titleJournal Water Science & Technology
Volume55
Issue8-9
Pages465471; # of pages: 7
Subjectenv; ultrastructure
AbstractThe electrolytical methanogenic/methanotrophic coupling (eMaMoC) process was tested in a laboratory-scale single-stage reactor for the treatment of tetrachloroethene (PCE)-contaminated waters. A water electrolysis cell was placed directly in the effluent recirculation loop for the supply of both O2 and H2 to the system: H2 serving as the electron donor for both carbonate reduction into CH4 and reductive dechlorination. The concurrent presence of O2 and CH4 could be used by the methanotrophs for co-metabolically oxidising the chlorinated intermediates left over by the anaerobic transformation of PCE. At a PCE inlet of 33-52 mM and a hydraulic residence time (HRT) of 5.6 days, PCE reductive dechlorination to dichloroethene (DCE) was over 95% with a maximum DCE mineralisation of 83%. Fluorescence in situ hybridisation with 16S rRNA probes related to type I and type II methanotrophic bacteria were utilised to localise the methanotrophic communities in the anaerobic/aerobic granules. It evidenced that with operational time, along with increasing oxygenation rate, methanotrophic communities were specifically colonising onto the outermost layer of the anaerobic/aerobic granule.
Publication date
LanguageEnglish
AffiliationNational Research Council Canada; NRC Biotechnology Research Institute
Peer reviewedNo
NRC number49031
NPARC number3539932
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Record identifierfe76a7d3-b94d-4cc9-9cc1-62acda732375
Record created2009-03-01
Record modified2016-05-09
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