Scattering density profile model of POPG bilayers as determined by molecular dynamics simulations and small-angle neutron and x-ray scattering experiments

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DOIResolve DOI: http://doi.org/10.1021/jp208920h
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TypeArticle
Journal titleThe Journal of Physical Chemistry B
Volume116
Issue1
Pages232239; # of pages: 8
AbstractWe combine molecular dynamics (MD) simulations and experiment, both small-angle neutron (SANS) and small-angle X-ray scattering (SAXS), to determine the precise structure of bilayers composed of 1-palmitoyl-2-oleoyl-sn-glycero-3-phosphatidylglycerol (POPG), a lipid commonly encountered in bacterial membranes. Experiment and simulation are used to develop a one-dimensional scattering density profile (SDP) model suitable for the analysis of experimental data. The joint refinement of such data (i.e., SANS and SAXS) results in the area per lipid that is then used in the fixed-area simulations. In the final step, the direct comparison of simulated-to-experimental data gives rise to the detailed structure of POPG bilayers. From these studies we conclude that POPG’s molecular area is 66.0 ± 1.3 Å2, its overall bilayer thickness is 36.7 ± 0.7 Å, and its hydrocarbon region thickness is 27.9 ± 0.6 Å, assuming a simulated value of 1203 Å3 for the total lipid volume.
Publication date
LanguageEnglish
AffiliationNRC Canadian Neutron Beam Centre; National Research Council Canada
Peer reviewedYes
NPARC number19577563
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Record identifierfe9f4ce3-99c4-4e01-9833-f85c64a21bbf
Record created2012-03-03
Record modified2016-05-09
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