Glycosylation of DsbA in Francisella tularensis subsp. Tularensis

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DOIResolve DOI: http://doi.org/10.1128/JB.00438-11
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TypeArticle
Journal titleJournal of Bacteriology
ISSN0021-9193
Volume193
Issue19
Pages54985509; # of pages: 12
Subjectbacterial protein; DsbA protein; hydronium ion; PilA protein; serine; threonine; unclassified drug; amino acid sequence; animal experiment; animal model; article; bacterial virulence; bacterium mutant; carboxy terminal sequence; controlled study; female; Francisella tularensis; gene locus; liquid chromatography; mouse; nonhuman; nucleotide sequence; priority journal; protein glycosylation; protein modification; protein structure; subcutaneous fat disorder; tandem mass spectrometry; tularemia; Animals; Bacterial Proteins; Electrophoresis, Gel, Two-Dimensional; Female; Francisella tularensis; Glycosylation; Mice; Mice, Inbred BALB C; Multigene Family; Reverse Transcriptase Polymerase Chain Reaction; Tularemia; Virulence; Virulence Factors; Francisella tularensis subsp. tularensis; Murinae
AbstractIn Francisella tularensis subsp. tularensis, DsbA has been shown to be an essential virulence factor and has been observed to migrate to multiple protein spots on two-dimensional electrophoresis gels. In this work, we show that the protein is modified with a 1,156-Da glycan moiety in O-linkage. The results of mass spectrometry studies suggest that the glycan is a hexasaccharide, comprised of N-acetylhexosamines, hexoses, and an unknown monosaccharide. Disruption of two genes within the FTT0789-FTT0800 putative polysaccharide locus, including a galE homologue (FTT0791) and a putative glycosyltransferase (FTT0798), resulted in loss of glycan modification of DsbA. The F. tularensis subsp. tularensis ΔFTT0798 and ΔFTT0791::Cm mutants remained virulent in the murine model of subcutaneous tularemia. This indicates that glycosylation of DsbA does not play a major role in virulence under these conditions. This is the first report of the detailed characterization of the DsbA glycan and putative role of the FTT0789-FTT0800 gene cluster in glycan biosynthesis. © 2011, American Society for Microbiology.
Publication date
LanguageEnglish
AffiliationNational Research Council Canada (NRC-CNRC); NRC Institute for Biological Sciences (IBS-ISB)
Peer reviewedYes
NPARC number21271529
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Record identifierff4ea084-87c7-4727-8697-482df059dacf
Record created2014-03-24
Record modified2016-05-09
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