A Quick Method for Purifying Bile Acid-Stimulated Lipases

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DOIResolve DOI: http://doi.org/10.1007/BF00159518
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TypeArticle
Journal titleBiotechnology Techniques
Volume10
Issue7
Pages523528; # of pages: 6
AbstractA simple, one step chromatography procedure of general utility for purifying bile salt-activated lipases (BAL; cholesterol esterases) using a cholate affinity column is described. A key feature of the method is the use of a taurocholate concentration gradient in the eluent. The method has been used successfully to purify bovine and porcine BAL from commercial crude sources and BAL from human milk whey.
Publication date
LanguageEnglish
AffiliationNational Research Council Canada; NRC Steacie Institute for Molecular Sciences
Peer reviewedNo
NPARC number12328608
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Record identifierffd14fc5-2c3c-4413-bc4e-ae143a072932
Record created2009-09-10
Record modified2016-05-09
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