Near-field scanning optical microscopy detects nanoscale glycolipid domains in the plasma membrane

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TypeArticle
Journal titleJournal Of Microscopy
Volume232
Issue2
Pages225234; # of pages: 10
Subjectconfocal microscopy; glycolipid; membrane rafts; near-field scanning optical microscopy
AbstractThe localization of asialo-GM1 in ordered membrane raft domains in HeLa cells has been examined using a combination of membrane fractionation and fluorescence imaging. The glycolipid is enriched in Triton X-100 insoluble membrane fractions that contain high concentrations of cholesterol and caveolin-1 but is also found in detergent soluble membrane fractions. Near-field fluorescence microscopy shows that a fraction of the asialo-GM1 is localized in small nanoscale clusters that have an upper limit for the average diameter of approximately 90 nm and are partially colocalized with caveolae membrane domains. In addition to clusters, a diffuse, non-clustered population of asialo-GM1 is observed and is hypothesized to correspond to glycolipid isolated in detergent soluble membrane fractions.
Publication date
LanguageEnglish
AffiliationNational Research Council Canada; NRC Institute for Biological Sciences; NRC Steacie Institute for Molecular Sciences
Peer reviewedNo
NRC numberABULROB2008B
NPARC number9381631
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Record identifierffee2abb-a5ea-4847-a530-083d118fb406
Record created2009-07-10
Record modified2016-05-09
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