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Strong muscle-specific regulatory cassettes based on multiple copies of the human slow troponin I gene upstream enhancer

NRC Institute for Research in Construction; NRC Biotechnology Research Institute; National Research Council Canada
Published in:
Human Gene Therapy
Pages :
NPArC #:
activity; bio; Biotechnology; Cell Culture; Cells; Cytomegalovirus; Dna; Gene Therapy; Human; I; immunology; Liver; Mice; microbiology; Protein; Proteins; Recombinant Proteins; Tail; therapy
Bioprocesses Development Program; Programme de développement de bioprocédés
Bioprocess Center; Centre Bioprocédés
High-level tissue-specific expression of recombinant proteins in muscle is an important issue for several therapeutic applications. To achieve this goal, we generated several constructs containing one to five copies of the upstream enhancer (USE) of 160-bp of the human slow troponin I gene, linked to that gene's minimal promoter. We also tested constructs made with one to four copies of a 100-bp deletion of USE (+ΔUSE) reported to drive pan-muscle-specific expression in transgenic mice. These constructs were evaluated by measuring the activity of the reporter gene +β-galactosidase (+β-gal). In cell culture, these multimerized enhancers retained tissue specificity and their transcriptional strength increased with the number of enhancer copies. In myotube cultures (which still contain nondifferentiated cells), constructs containing four and five USE copies were stronger than the cytomegalovirus (CMV) early enhancer/promoter and slightly weaker than the hybrid CMV enhancer/+β-actin (CB) promoter. Those containing three USE, or four +(ΔUSE) copies were similar in strength to CMV. After electrotransfer of plasmid DNA into the mouse tibialis anterior muscle, the strengths of the two constructs (USEx3 and +ΔUSEx3) were tested; as measured by +β-gal activity in the total muscle lysate and by the number of transduced fibers, they were similar to CMV and CB. Muscle fiber typing, after electrotransfer of the soleus muscle, showed that +ΔUSEx3 and USEx3 were active in slow and fast fibers. The tissue specificity of these two constructs was also evaluated by hydrodynamic plasmid injection through the tail vein. Although significant +β-gal expression was measured in the liver when CMV was tested, no expression above background level was detected with USEx3 and +ΔUSEx3. The strength, muscle specificity, and small size of these transcriptional elements render them very attractive for gene therapy applications.
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