Government of Canada
Symbol of the Government of Canada

Common menu bar links

The NRC Publications Archive is now operational; however, not all the features of the site are available at this time.

The following features remain unavailable:

  • Viewing/Downloading of full text publications

NRC is currently working to restore these features and we will update this notice as these features become available. Thank you for your patience.


The influence of pore size on osteoblast phenotype expression in cultures grown on porous titanium

NRC Industrial Materials Institute
Published in:
International Journal of Oral and Maxillofacial Surgery
Pages :
NPArC #:
cell culture; osteoblast; dental implant; pore size; porous titanium
This study investigated the effect of pore size on osteoblastic phenotype development in cultures grown on porous titanium (Ti). Porous Ti discs with three different pore sizes, 312 μm (Ti 312), 130 μm (Ti 130) and 62 μm (Ti 62) were fabricated using a powder metallurgy process. Osteoblastic cells obtained from human alveolar bone were cultured on porous Ti samples for periods of up to 14 days. Cell proliferation was affected by pore size at day 3 (p = 0.0010), day 7 (p = 0.0005) and day 10 (p = 0.0090) in the following way: Ti 62 < Ti 130 < Ti 312. Gene expression of bone markers evaluated at 14 days was affected, RUNX2 (p = 0.0153), ALP (p = 0.0153), BSP (p = 0.0156), COL (p = 0.0156), and OPN (p = 0.0156) by pore size as follows: Ti 312 < Ti 130 < Ti 62. Based on these results, the authors suggest that porous Ti surfaces with pore sizes near 62 μm, compared with those of 312 μm and 130 μm, yield the highest expression of osteoblast phenotype as indicated by the lower cell proliferation rate and higher gene expression of bone markers.
Bookmark and Share:
HTML Link: