Separation and characterization of alkaloids in goldenseal (Hydrastis canadensis) by ultra-performance liquid chromatography coupled with electrospray ionization quadruple time of flight mass spectrometry (UPLC/Q-TOF-MS)

Alternative titleApplication of UPLC-QTOF-MS in MSE mode for the rapid and precise identification of alkaloids in goldenseal (Hydrastis canadensis)
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DOIResolve DOI: http://doi.org/10.1007/s00216-013-7558-x
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TypeArticle
Journal titleAnalytical and Bioanalytical Chemistry
ISSN1618-2642
Pages111; # of pages: 11
AbstractHere, we describe a new application of ultra-performance liquid chromatography coupled with an electrospray ionization quadrupole time-of-flight mass spectrometry operating in MSE mode (UPLC-QTOF-MSE) for the sensitive, fast, and effective characterization of alkaloids in goldenseal (Hydrastis canadensis). This approach allowed identification of alkaloids using a cyclic low and high collision energy spectral acquisition mode providing simultaneous accurate precursor and fragment ion mass information. A total of 45 compounds were separated and 40 of them characterized including one new compound and 7 identified for the first time in goldenseal. The spectral data obtained using this method is comparable to those obtained by conventional LC-MSn. However, the UPLC-QTOF-MSE method offers high chromatographic resolution with structural characterization facilitated by accurate mass measurement in both MS and MS/MS modes in a single analytical run; this makes it suitable for the rapid analysis and screening of alkaloids in plant extracts. [Figure not available: see fulltext.] © 2014 Her Majesty the Queen in Right of Canada.
Publication date
LanguageEnglish
AffiliationNational Research Council Canada (NRC-CNRC); Measurement Science and Standards (MSS-SME)
Peer reviewedYes
NPARC number21270802
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Record identifier0caece70-6f53-4350-bf02-8e7625264f7a
Record created2014-02-17
Record modified2016-05-09
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