Determination of cyanocobalamin by isotope dilution LC-MS/MS

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DOIResolve DOI: http://doi.org/10.1016/j.aca.2017.07.065
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TypeArticle
Journal titleAnalytica Chimica Acta
ISSN0003-2670
Volume990
Pages103109
Subjectcyanocobalamin; isotope dilution; liquid chromatography mass spectrometry; multivitamins
AbstractCyanocobalamin (CNCbl) is an active form of vitamin B12, commonly employed for the preparation of multivitamin supplements and fortified food. In this study, we present a novel analytical method for its determination based on stable isotope dilution liquid chromatography electrospray tandem mass spectrometry (ID LC-MS/MS). Isotopically enriched 13C15NCbl was synthesized in-house and used as internal standard. The method was validated using NIST SRM 3280 multivitamin reference material and by comparison with an independent methodology based on LC-ICPMS. The proposed method provided a detection limit of 57 pg/g and could be applied for the determination of trace level of CNCbl in multivitamin supplements with a relative standard uncertainty better than 3%. The novel ID LC-MS/MS is a primary ratio method that could become a reference for CNCbl determination in multivitamins and food supplements. The method was applied for the characterization of two NRC multivitamin tablet Certified Reference Material (CRM) candidates, VITA-1 and VITB-1 whose CNCbl levels were quantified as 2.64 ± 0.09 and 1.75 ± 0.12 μg/g, respectively.
Publication date
PublisherElsevier
LanguageEnglish
AffiliationMeasurement Science and Standards; Security and Disruptive Technologies; National Research Council Canada
Peer reviewedYes
NPARC number23002343
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Record identifier0e50af11-8f2e-4928-89a0-41808abde647
Record created2017-10-20
Record modified2017-10-20
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