The structure of the extracellular teichoic acids from the allergy-protective bacterium Lactococcus lactis G121

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DOIResolve DOI: http://doi.org/10.1515/hsz-2012-0142
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TypeArticle
Journal titleBiological Chemistry
ISSN1431-6730
Volume393
Issue8
Pages749755; # of pages: 7
Subjectantiallergic agent; extracellular teichoic acid; sodium chloride; teichoic acid; unclassified drug; allergic asthma; article; chemical structure; controlled study; electrospray mass spectrometry; extraction; gel permeation chromatography; ion cyclotron resonance mass spectrometry; Lactococcus lactis; nonhuman; priority journal; proton nuclear magnetic resonance; Animals; Carbohydrate Sequence; Hypersensitivity; Lactococcus lactis; Mice; Molecular Sequence Data; Nuclear Magnetic Resonance, Biomolecular; Spectrometry, Mass, Electrospray Ionization; Teichoic Acids
AbstractThe Gram-positive bacterium Lactococcus lactis G121 is a farm isolate that protects mice from ovalbumin-induced asthma. To understand the molecular mechanisms of such allergy-protective properties, the isolation and characterization of cell envelope constituents is crucial. Here, structural analyses of the extracellular teichoic acid (EC TA) from L. lactis G121 are presented. Extraction with 0.9 % saline afforded a crude TA fraction. Consecutive size exclusion chromatography on Biogel P60 and P10 matrix was performed to purify the sample. Chemical component analyses, high-resolution electrospray ionization Fourier-transformed ion cyclotron mass spectrometry, and nuclear magnetic resonance spectroscopy were conducted for structural elucidation. The EC TA was a poly(glycosylglycerol phosphate) molecule with a repeating unit of -6)-[β- d-Glcp -(1→3)-][α- d-Glcp NAc-(1→4)-]α- d-Galp NAc-(1→3)-β-d-Glcp NAc-(1→2)-glycerol-(1- P -). Copyright © by Walter de Gruyter • Berlin • Boston.
Publication date
LanguageEnglish
AffiliationNational Research Council Canada (NRC-CNRC); NRC Institute for Biological Sciences (IBS-ISB)
Peer reviewedYes
NPARC number21269410
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Record identifier1463305d-cd2d-4694-97d6-5be431f56e7f
Record created2013-12-12
Record modified2016-05-09
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