Defects in myoglobin oxygenation in KATP-deficient mouse hearts under normal and stress conditions characterized by near infrared spectroscopy and imaging

Download
  1. (PDF, 515 KB)
  2. Get@NRC: Defects in myoglobin oxygenation in KATP-deficient mouse hearts under normal and stress conditions characterized by near infrared spectroscopy and imaging (Opens in a new window)
DOIResolve DOI: http://doi.org/10.1016/j.ijcard.2010.02.009
AuthorSearch for: ; Search for: ; Search for: ; Search for: ; Search for: ; Search for:
TypeArticle
Journal titleInternational Journal of Cardiology
Volume149
Issue3
Pages315322; # of pages: 8
SubjectNear infrared spectroscopic imaging; Kir6.2 knockout; Langendorff-perfused mouse heart; Heterogeneity of myoglobin oxygen saturation; 2,4-dinitrophenol; Isoproterenol
AbstractBackground: Disruption of ATP-sensitive potassium (KATP) channel activity results in the development of dilated cardiomyopathy in response to different forms of stress, likely due to the underlying metabolic defects. To further understand the role of Kir6.2-containing channels in the development of cardiac disease, we analysed the left ventricular (LV) wall oxygenation and the physiologic responses induced by acute stress in non-dilated Kir6.2−/− hearts. Methods: Control (C57BL6) and Kir6.2−/− mouse hearts were perfused in constant flow Langendorff mode with Krebs–Henseleit buffer. Myocardial oxygenation was evaluated using a newly developed technique, near infrared spectroscopic imaging (NIRSI) of the myoglobin (Mb) oxygen saturation parameter (OSP, ratio of oxy- to total Mb). Results: 2,4-dinitrophenol (DNP, 50-µM) and isoproterenol (0.1-µM) failed to produce a transient vasodilatory response and caused a significant diastolic pressure increase in Kir6.2−/− hearts. DNP strongly suppressed contractile function in both groups and induced severe mean OSP decreases in Kir6.2−/− hearts. Isoproterenol-induced decreases in OSP were similar despite the lack of contractile function stimulation in the Kir6.2−/− group. The index of OSP spatial heterogeneity (relative dispersion, RD) was lower by 15% in the Kir6.2−/− group at the baseline conditions. Recovery after stress caused reduction of RD values by 20% (DNP) and 8% (isoproterenol) in controls; however, these values did not change in the Kir6.2−/− group. Conclusions: 1) NIRSI can be used to analyse 2-D dynamics of LV oxygenation in rodent models of cardiomyopathy; 2) Kir6.2-containing KATP channels play an important role in maintaining myocardial oxygenation balance under acute stress conditions and in post-stress recovery.
Publication date
LanguageEnglish
AffiliationNRC Institute for Biodiagnostics; National Research Council Canada
Peer reviewedYes
NPARC number17653080
Export citationExport as RIS
Report a correctionReport a correction
Record identifier31e42bc7-7fa3-4b52-b70f-ca0b8addeb1e
Record created2011-04-03
Record modified2016-05-09
Bookmark and share
  • Share this page with Facebook (Opens in a new window)
  • Share this page with Twitter (Opens in a new window)
  • Share this page with Google+ (Opens in a new window)
  • Share this page with Delicious (Opens in a new window)