Detection of acute brain injury by Raman spectral signature

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Journal titleAnalyst
Pages16201626; # of pages: 7
Subjectamide; caspase 3; animal; article; brain injury; chemistry; discriminant analysis; enzymology; metabolism; methodology; mouse; pathology; principal component analysis; Raman spectrometry; Amides; Animals; Brain Injuries; Caspase 3; Discriminant Analysis; Mice; Principal Component Analysis; Spectrum Analysis, Raman
AbstractBrain injury can lead to irreversible tissue loss and functional deficit along with significant health care costs. Raman spectroscopy can be used as a non-invasive technique to provide detailed information on the molecular composition of diseased and damaged tissues. This technique was used to examine acute mouse brain injury, focusing on the motor cortex, a region directly involved in controlling execution of movement. The spectral profile obtained from the injured brain tissue revealed a markedly different signature, particularly in the amide I and amide III vibrational region when compared to that of healthy brain tissue. Most noticeably, there was a significant reduction of the amide I vibration at the acute injury site and the appearance of two distinct features at 1586 and 1618 cm -1. Complementary immunohistochemical analysis of the injured brain tissue showed an abundant expression of Caspase 3 (a cysteine protease marker used for apoptosis), suggesting that the injury-induced specific Raman shifts may be correlated with cell death. Taken together, this study demonstrates that Raman spectroscopy can play an important role in detecting the changes that occur in the injured brain and provide a possible technology for monitoring the recovery process. © 2011 The Royal Society of Chemistry.
Publication date
AffiliationNational Research Council Canada (NRC-CNRC); NRC Institute for Microstructural Sciences (IMS-ISM); NRC Institute for Biological Sciences (IBS-ISB)
Peer reviewedYes
NPARC number21271039
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Record identifier4769f3fa-1dd9-46a1-902d-2de53ab0ce8d
Record created2014-03-24
Record modified2016-05-09
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