Quantitative high-throughput analysis of domoic acid in mussel tissue homogenates using laser ablation electrospray ionization – MS/MS

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TypePresentation
Conference30th Montreaux LC/MS Symposium, 3–7 November 2014, Charleston, USA
AbstractWe investigated the use of Laser Ablation Electrospray Ionization (LAESI) with MS/MS detection for Domoic Acid (DA) analysis directly from mussel tissue homogenates without sample extraction, cleanup or chromatographic separation. DA could be selectively detected directly from mussel tissue homogenates using LAESI-MS/MS in selected reaction monitoring scan mode. A matrix matched calibration curve was used to quantify mussel tissue reference materials and results were in good agreement with two established methods, LC-UV and LC-MS/MS, within the linear range. Most notable is the decrease in run time from about 20 minutes for a typical chromatographic method to about 10 seconds per sample for LAESI-MS/MS.
Publication date
LanguageEnglish
AffiliationNational Research Council Canada; Measurement Science and Standards
Peer reviewedYes
NPARC number23002022
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Record identifier8a18e683-ac07-4502-a1f5-e6d97c095119
Record created2017-07-21
Record modified2017-07-25
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