DNA repeats identify novel virulence genes in Haemophilus influenzae: Proc Natl Acad Sci U.S.A.

Download
  1. Get@NRC: DNA repeats identify novel virulence genes in Haemophilus influenzae: Proc Natl Acad Sci U.S.A. (Opens in a new window)
AuthorSearch for: ; Search for: ; Search for: ; Search for: ; Search for: ; Search for: ; Search for:
TypeArticle
Journal titleProc Natl Acad Sci U.S.A.
Volume93
Issue20
Pages1112111125; # of pages: 5
SubjectAnimal; Antigenic Variation; Antigens,Bacterial; Bacteria; DNA,Bacterial; Genes,Structural,Bacterial; Glycosyltransferases; Haemophilus; Haemophilus influenzae; LIPOPOLYSACCHARIDE; Neisseria; Open Reading Frames; Polysaccharides,Bacterial; Rats; Repetitive Sequences,Nucleic Acid; Sequence Analysis; Support,Non-U.S.Gov't; Virulence
AbstractThe whole genome sequence (1.83 Mbp) of Haemophilus influenzae strain Rd was searched to identify tandem oligonucleotide repeat sequences. Loss or gain of one or more nucleotide repeats through a recombination-independent slippage mechanism is known to mediate phase variation of surface molecules of pathogenic bacteria, including H. influenzae. This facilitates evasion of host defenses and adaptation to the varying microenvironments of the host. We reasoned that iterative nucleotides could identify novel genes relevant to microbe-host interactions. Our search of the Rd genome sequence identified 9 novel loci with multiple (range 6-36, mean 22) tandem tetranucleotide repeats. All were found to be located within putative open reading frames and included homologues of hemoglobin-binding proteins of Neisseria, a glycosyltransferase (IgtC gene product) of Neisseria, and an adhesin of Yersinia. These tetranucleotide repeat sequences were also shown to be present in two other epidemiologically different H. influenzae type b strains, although the number and distribution of repeats was different. Further characterization of the IgtC gene showed that it was involved in phenotypic switching of a lipopolysaccharide epitope and that this variable expression was associated with changes in the number of tetranucleotide repeats. Mutation of IgtC resulted in attenuated virulence of H. influenzae in an infant rat model of invasive infection. These data indicate the rapidity, economy, and completeness with which whole genome sequences can be used to investigate the biology of pathogenic bacteria Type: JOURNAL ARTICLE Language: Eng
Publication date
LanguageEnglish
AffiliationNRC Institute for Biological Sciences; National Research Council Canada
Peer reviewedNo
NRC numberHOOD1996B
NPARC number9362480
Export citationExport as RIS
Report a correctionReport a correction
Record identifier9e9734f3-b0bb-4b3b-b4ae-c3c3e05a13d8
Record created2009-07-10
Record modified2016-05-09
Bookmark and share
  • Share this page with Facebook (Opens in a new window)
  • Share this page with Twitter (Opens in a new window)
  • Share this page with Google+ (Opens in a new window)
  • Share this page with Delicious (Opens in a new window)