Boron doped diamond biosensor for detection of Escherchia coli

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DOIResolve DOI: http://doi.org/10.1021/jf8013958
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TypeArticle
Journal titleJournal of Agricultural and Food Chemistry
Volume56
Issue17
Pages76917695; # of pages: 5
SubjectENV; electrochemical detection; boron doped diamond; E. coli; β-galactosidase; ο-nitrophenyl-β-D-galactophyranose
Abstractο-Nitrophenol, released from ο-nitrophenyl-β-D-galactopyranose as catalyzed by β-galactosidase, a tetramer of Escherichia coli, has been exploited for the detection of E. coli contamination in foodstuffs. This reaction was detected using a boron doped diamond electrode poised at +0.93 V, without any surface modification. The enzyme was effectively induced by isopropyl-β-D-thiogalacto-pyranoside with the maximum enzyme activity observed with sodium dodecyl sulfate at 50 °C. A biphasic calibration plot was observed: 4 × 10⁴ to 2 × 10⁵ cells/mL and 2 × 10⁵ to 6 × 10⁶ cells/mL for the first and second region, respectively. The detection limit was 4 × 10⁴ cells/mL with a total analysis time of <1.5 h. Electrode fouling was easily overcome by ∼40 rapid CV scans, and the method was applicable for detecting E. coli in artificially spiked samples of beef, pork, chicken, milk, and tap water.
Publication date
LanguageEnglish
AffiliationNational Research Council Canada; NRC Biotechnology Research Institute
Peer reviewedNo
NRC number49944
NPARC number12455738
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Record identifiercc7a9a6f-62eb-4bd3-8820-711812b04a40
Record created2009-10-26
Record modified2016-05-09
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