Prohormone processing in Zebrafish

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Journal titleMethods in Molecular Biology
Pages257271; # of pages: 15
Subjectmessenger RNA; protein precursor; serine proteinase; zebrafish protein; Alzheimer disease; animal; animal embryo; article; chemistry; DNA sequence; enzymology; gene silencing; genetics; human; infection; metabolism; methodology; morphogenesis; neoplasm; tissue distribution; transgenic animal; zebra fish; Alzheimer Disease; Animals; Animals, Genetically Modified; Embryo, Nonmammalian; Gene Knockdown Techniques; Humans; Infection; Morphogenesis; Neoplasms; Proprotein Convertases; Protein Precursors; RNA, Messenger; Sequence Analysis, DNA; Tissue Distribution; Zebrafish; Zebrafish Proteins; Danio rerio
AbstractProprotein convertases (PCs) are secretory proteolytic enzymes that activate precursor proteins into biologically active forms by limited proteolysis at one or multiple internal sites. PCs are implicated in the processing of multiple protein precursors, including hormones, proteases, growth factors, angiogenic factors, and receptors. PCs have been linked recently to various pathologies such as Alzheimer's disease, tumorigenesis, and infections. The zebrafish has emerged as an attractive model for studying the role of PCs not only in substrate production but also in development. Herein we describe methods that are used to characterize DNA sequences of PCs in zebrafish, as well as to evaluate the ontogeny and tissue distribution of their transcripts. We also provide information on the morpholino-mediated knockdown of proprotein convertases. © 2011 Springer Science+Business Media, LLC.
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AffiliationNational Research Council Canada (NRC-CNRC); NRC Institute for Marine Biosciences (IMB-IBM)
Peer reviewedYes
NPARC number21271398
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Record identifierccd307d7-5337-4234-853c-336c5fd71b5d
Record created2014-03-24
Record modified2016-05-09
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