Accelerated mass production of influenza virus seed stocks in HEK-293 suspension cell cultures by reverse genetics

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DOIResolve DOI: http://doi.org/10.1016/j.vaccine.2017.04.065
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TypeArticle
Journal titleVaccine
ISSN0264-410X
Volume35
Issue26
Pages34233430
Subjectcandidate Vaccine Virus (CVV); influenza virus; large-scale; pandemic; reverse genetics; suspension cell culture; transient transfection; vaccines
AbstractDespite major advances in developing capacities and alternative technologies to egg-based production of influenza vaccines, responsiveness to an influenza pandemic threat is limited by the time it takes to generate a Candidate Vaccine Virus (CVV) as reported by the 2015 WHO Informal Consultation report titled "Influenza Vaccine Response during the Start of a Pandemic". In previous work, we have shown that HEK-293 cell culture in suspension and serum free medium is an efficient production platform for cell culture manufacturing of influenza candidate vaccines. This report, took advantage of, recombinant DNA technology using Reverse Genetics of influenza strains, and advances in the large-scale transfection of suspension cultured HEK-293 cells. We demonstrate the efficient generation of H1N1 with the PR8 backbone reassortant under controlled bioreactor conditions in two sequential steps (transfection/rescue and infection/production). This approach could deliver a CVV for influenza vaccine manufacturing within two-weeks, starting from HA and NA pandemic sequences. Furthermore, the scalability of the transfection technology combined with the HEK-293 platform has been extensively demonstrated at >100L scale for several biologics, including recombinant viruses. Thus, this innovative approach is better suited to rationally engineer and mass produce influenza CVV within significantly shorter timelines to enable an effective global response in pandemic situations.
Publication date
PublisherElsevier
LanguageEnglish
AffiliationHuman Health Therapeutics; National Research Council Canada
Peer reviewedYes
NPARC number23002171
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Record identifiercfbf971a-e649-47f9-9357-a6501636ecd3
Record created2017-08-29
Record modified2017-08-29
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