Functional dissection of human protease μ-calpain in cell migration using RNAi

Download
  1. Get@NRC: Functional dissection of human protease μ-calpain in cell migration using RNAi (Opens in a new window)
DOIResolve DOI: http://doi.org/10.1016/j.febslet.2006.05.003
AuthorSearch for: ; Search for: ; Search for: ; Search for: ; Search for: ; Search for:
TypeArticle
Journal titleFEBS Letters
ISSN0014-5793
Volume580
Issue13
Pages32463256; # of pages: 11
SubjectCalpain; Cell Movement; Cells, Cultured; Contractile Proteins; Humans; Microfilament Proteins; RNA Interference; RNA, Small Interfering; Talin
AbstractCalpains are a family of calcium-dependent cysteine proteases involved in a variety of cellular functions. Two isoforms, m-calpain and μ-calpain, have been implicated in cell migration. However, since conventional inhibitors used for the studies of the functions of these enzymes lack specificity, the individual physiological function and biochemical mechanism of these two isoforms, especially μ-calpain, are not clear. In contrast, RNA interference has the potential to allow a sequence-specific destruction of target RNA for functional assay of gene of interest. In the present study, we found that small interfering RNAs-mediated knockdown of μ-calpain expression in MCF-7 cells that do not express m-Calpain led to a reduction of cell migration. This isoform-specific function of μ-calpain was further confirmed by the rescue experiment as overexpression of μ-calpain but not m-calpain could restore the cell migration rate. Knockdown of μ-calpain also altered cell morphology with increased filopodial projections and a highly elongated tail that seemed to prevent cell spreading and migration with reduced rear detachment ability. Furthermore, knockdown of μ-calpain decreased the proteolytic products of filamin and talin, which were specifically rescued by overexpression of μ-calpain but not m-calpain, suggesting that their proteolysis could be one of the key mechanisms by which μ-calpain regulates cell migration.
Publication date
LanguageEnglish
AffiliationNational Research Council Canada; NRC Biotechnology Research Institute
Peer reviewedNo
Identifier10270292
NPARC number12328316
Export citationExport as RIS
Report a correctionReport a correction
Record identifierfe62fa8f-0b4e-403c-a60a-86688a667b55
Record created2009-09-10
Record modified2016-05-09
Bookmark and share
  • Share this page with Facebook (Opens in a new window)
  • Share this page with Twitter (Opens in a new window)
  • Share this page with Google+ (Opens in a new window)
  • Share this page with Delicious (Opens in a new window)